The release kinetic values of regression coefficients confirmed the diffusion-dependent release of the drug. Proliposomal gel showed the prolonged release of FLZ than the lyophilized liposomes. The FTIR and DSC studies showed no possible drug-excipient interaction. The percentage entrapment of drug was increased with increase in phospholipid composition in the range of 55.13–69.61%. Topical proliposomal gels were prepared by incorporation of lyophilized proliposome into a structured vehicle carbopol 934 (2.5%).Results: A spherical shape of reconstituted FLZ liposome with an average vesicle about 5–8 μm was observed in photomicrographs. Proliposome formulations were characterized for vesicle size, vesicle size distribution, vesicle morphology, drug content, entrapment efficiency, percentage yield value, storage stability analysis, Fourier-transform infrared spectroscopy (FTIR), differential scanning calorimetry (DSC), in vitro diffusion study, release kinetic studies, and antifungal activity. Objectives: The present objective for the study was to prepare proliposomal gel bearing an antifungal agent, fluconazole (FLZ) intended for topical application.Methods: Various proliposome formulations were prepared using thin-film hydration technique by varying the lipid phase composition (phosphatidylcholine/cholesterol). Conclusion: The formulation of the liposomal delivery system of heme protein was successfully prepared using natural components and evaluated for different parameters. Cyt-LS exhibit specific activity, similar to non-liposomal Cyt-C solution. Also, the size of Cyt-LS particles was confirmed by the ability of emulsion subjected to the sterilizing filtration with the preservation of its main physicochemical properties. The average particle diameter was 156☒ nm. #MOTIC IMAGE PLUS DOWNLOAD FREE#Phospholipid impurities had the following content: lysophosphatidylcholine-0.60☐.05% and free fatty acids-0.4☐.05%. The obtained Cyt-LS were characterized by the main physicochemical parameters showed: Encapsulation efficiency 95.8☒.0%, Zeta potential-57☑.0 mV, pH-6.95☐.05. The selected temperature regime of homogenization was kept within 38–44 °С with optimal homogenization pressure of 800 bar. Results: The study of homogenization regimes for obtaining unilamellar Cyt-LS was carried out. The specific activity was studied in vitro. Nanoparticles were characterized by using: dynamic light scattering, zeta potential measurements, scanning electron microscopy and HPLC. Methods: Cytochrome C containing liposomes (Cyt-LS) were prepared by high-pressure homogenization technique using phosphatidylcholine (PC) and dipalmitoyl phosphatidylglycerol (DPPG). Objective: The objective of the present study was to develop and optimize the methods for preparation and characterization of the liposomal delivery system of natural heme protein. No changes were founded during the short-term stability study of F4. Conclusion: The F4 batches showed promising results compared to other formulations. Short term stability study of formulation F4 showed no significant change in release kinetics. F4 formulation was found to follow significantly zero-order release kinetics. F4 shows highest release of about 79.0% in 4 h. The drug entrapment efficiency of all batches was found within the range of 51.42 % to 79.0 %. Results: F2 and F9formulations showed better drug entrapment efficiency compared to the other batches. Stability study was carried out with F4 batch. All batches were evaluated by drug entrapment and release kinetic studies. Total nine batches were prepared and each batch was prepared in triplicate. Mixture of phosphotidyl choline (soya lecithin), acyclovir and cholesterol of varying weight ratio was used for the preparation of liposomes. Methods: Liposomal formulation of acyclovir was prepared by “thin lipid film method”. Objective: The objective of this study was to formulate and evaluate liposomes loaded with acyclovir.
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